Integrated Microfluidic Chip Design for Nucleic Acid Detection and Analysis of Droplet Manipulation
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摘要: 设计了一种基于磁珠法的两相流液滴微流控芯片，包含样品提纯、扩增和检测一系列连续的生化过程，可用于快速核酸检测。首先建立了磁珠和液滴在两相流体系内的动力学模型，并借助有限元仿真平台对磁珠微团所处环境进行了磁场仿真，得到不同体积磁珠微团所受的磁力。进一步还分析了磁珠微团、液滴体积、永磁体移动速度对液滴运动状态的影响，最终总结出液滴操纵图。Abstract: A two-phase flow droplet microfluidic chip based on magnetic bead method is designed in this paper, which includes a series of continuous biochemical processes for sample purification, amplification and detection, and this chip can be used for rapid nucleic acid detection. First, the dynamic models of magnetic beads and droplets in a two-phase flow system were established, and a magnetic field simulation was performed on the environment of the magnetic bead micelles by means of a finite element simulation platform to obtain the magnetic forces on the magnetic bead micelles of different volumes. Secondly, the three movement states of droplets of different volumes under the action of different volume of magnetic bead micelles are analyzed. Finally the relationship between the magnetic bead micelles, droplet volume and droplet movement is analyzed, and the droplet manipulation diagram is summarized.
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表 1 物理参数值
参数名 参数值 永磁体底面直径⌀/mm 1.5 永磁体高度h/mm 10 永磁体X方向剩余磁通密度Br/mT 0 永磁体Y方向剩余磁通密度Br/mT 0 永磁体Z方向剩余磁通密度Br/mT 500 真空磁导率μ0/(H·m-1) 4π×10-7 磁珠体积磁化率χ 0.29 油/水界面张力/(N·m-1) 0.051 磁珠微团球心距永磁体端部距离/mm 2 接触角θ/(°) 160 硅油粘度μoil/(Pa·s) 0.05 
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