一步成形法制备单细胞培养芯片 -- 西北工业大学学报,2018,36(1):132-138
论文:2018,Vol:36,Issue(1):132-138
引用本文:
叶芳, 何美莹, 谢晋, 谢丽, 常洪龙, 苑伟政. 一步成形法制备单细胞培养芯片[J]. 西北工业大学学报
Ye Fang, He Meiying, Xie Jin, Xie Li, Chang Honglong, Yuan Weizheng. A Novel Single Cell Culture Chip Based on One-Step Method[J]. Northwestern polytechnical university

一步成形法制备单细胞培养芯片
叶芳1,2, 何美莹1,2, 谢晋3, 谢丽4,5, 常洪龙1,2, 苑伟政1,2
1. 西北工业大学 机电学院, 陕西 西安 710072;
2. 空天微纳系统教育部重点实验室, 陕西 西安 710072;
3. 中国工程物理研究院电子工程研究所, 四川 绵阳 621000;
4. 西北工业大学 生命学院, 陕西 西安 710072;
5. 空间生物实验模拟技术国防重点学科实验室, 陕西 西安 710072
摘要:
越来越多的生物学研究聚焦在单细胞层面,然而现有基于化学表面修饰的单细胞定位培养芯片存在制备工艺复杂,且细胞定位层图形质量较差影响细胞定位效果的问题。为此,提出一种一步成形的方法,利用聚甲基丙烯酸2-羟乙酯(poly 2-hydroxyethyl methacrylate,PolyHEMA)抑制细胞粘附的特性并结合毛细微模塑技术制备芯片。细胞验证结果表明,该芯片具有制备过程简单,图形完整性和稳定性良好的特点,为基于单细胞的生物学研究提供了一种新方法。
关键词:    单细胞培养    图形化    毛细微模塑技术    PolyHEMA   
A Novel Single Cell Culture Chip Based on One-Step Method
Ye Fang1,2, He Meiying1,2, Xie Jin3, Xie Li4,5, Chang Honglong1,2, Yuan Weizheng1,2
1. School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an 710072, China;
2. Key Laboratory of Micro/Nano Systems for Aerospace, Ministry of Education, Northwestern Polytechnical University, Xi'an 710072, China;
3. Institute of Electronic Engineering, China Academy of Engineering Physics, Mianyang 621000, China;
4. School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China;
5. Key Laboratory of Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, Northwestern Polytechnical University, Xi'an 710072, China
Abstract:
More and more basic biology research focus on the single cell level. However, the way to fabricate a chemically patterned surface exist some questions such as complex fabrication process, graphic quality puzzle and so on. In this paper, we present a One-Step method based on Poly 2-hydroxyethyl methacrylate (PolyHEMA) and micromolding in capillaries (MIMIC) technology to fabricate single cell culture chip. Experimental evidence indicates that the integrity and stability of the PolyHEMA pattern can perfectly satisfy the need of long-term cell culture.
Key words:    single cell culture    micropattern    MIMIC    PolyHEMA   
收稿日期: 2017-05-12     修回日期:
DOI:
基金项目: 国家自然科学基金(51375398)、国家重大仪器设备开发专项(2013YQ190467)、111引智项目(B13044)以及陕西省科技统筹创新工程重点实验室项目资助
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作者简介: 叶芳(1969-),女,西北工业大学副教授、博士,主要从事生物微机电系统及生物芯片技术研究。
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